Alain HEDOUX

Professeur

Bâtiment P5 - 253
Unité Matériaux et Transformations
Bât. P5
Université Lille1
59655 Villeneuve d'Ascq
France
Tel: +33 (0)3 20 43 46 77
Fax: +33 (0)3 20 43 40 84
Courriel: alain.hedoux@univ-lille1.fr

Equipe: Matériaux moléculaires et thérapeutiques

Recherche

Analyse de l’organisation structurale et de la dynamique des matériaux moléculaires formateurs de verre dans leurs états amorphes et cristallins, des mécanismes de transformation de phases, et de stabilisation d’états amorphes de matériaux d’intérêt thérapeutique (petites molécules, protéines) par des excipients. Analyses par spectroscopie Raman, diffusion des neutrons, RX, calorimétrie.

Enseignement

IUT A – département Mesures Physique : Mécanique du point, du solide indéformable, déformable (élasticité) Master : Organisation structurale des états condensés de la matière.

Thèses en cours

• Benjamin MALFAIT, Amélioration de la stabilité et de la biodisponibilité de matériaux pharmaceutiques et de biopharmaceutiques par confinement dans des systèmes à libération controlée . Thèse débutée en 2016. Co-directeur: Natalia Correia.

Thèses soutenues

• Tatiana Starciuc, Stabilisation de matériaux biomoléculaires pour applications pharmaceutiques. Thèse soutenue en 2017.
• Sarah Nasser, Évolution des propriétés structurales et fonctionnelles des poudres de PhosPho Caséinate Native (PPCN) en lien avec l’état initial et les conditions de vieillissement. Thèse soutenue en 2017. Co-directeur: Guillaume DELAPLACE.
• Samira Achir, Etude des mécanismes de stabilisation des protéines par spectroscopie Raman et dynamique moléculaire . Thèse soutenue en 2014. Co-directeur: Frédéric Affouard. Situation actuelle: Scientific officer chez Springwave - Algama SAS.
• Adrien Lerbret, Étude de l'action bioprotectrice des sucres : une investigation par dynamique moléculaire et spectroscopie Raman. Thèse soutenue en 2005. Co-directeurs: Frédéric Affouard, Marc Descamps. Situation actuelle: Ingénieur de Recherche - Université de Bourgogne-AgroSup Dijon.
• Thomas Denicourt, Etude spectroscopique de systèmes désordonnés profondément sous-refroidis . Thèse soutenue en 2002. Co-directeur: Marc Descamps. Situation actuelle: Responsable informatique.

Communications et séminaires récents

28 communication(s) orale(s), dont 13 invité(e)s. 11 communication(s) par affiche. Liste détaillée.

Chapitres d'ouvrage

1. A. Hedoux, in Disordered Pharmaceutical Materials [ed M. Descamps], Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, 361-392 (2016) [doi: 10.1002/9783527652693.ch12]

Publications dans des revues internationales

• 2018
  58. S. Nasser, A. Hédoux, A. Giuliani, C. Le Floch-Fouéré, V. Santé-Lhoutellier, I. De Waele, G. Delaplace, Investigation of secondary structure evolution of micellar casein powder upon aging by FTIR and SRCD: consequences on solubility, Journal of the Science of Food and Agriculture 98 2243-2250 (2018) [doi: 10.1002/jsfa.8711]
  57. Y. Karrout, F. Siepmann, Y. Benzine, L. Paccou, Y. Guinet, A. Hedoux, J. Siepmann, When drugs plasticize film coatings: Unusual formulation effects observed with metoprolol and Eudragit RS, International Journal of Pharmaceutics 539 39-49 (2018) [doi: 10.1016/j.ijpharm.2018.01.014]
• 2017
  56. S. Nasser, A. Moreau, R. Jeantet, A. Hédoux, G. Delaplace, Influence of storage conditions on the functional properties of micellar casein powder, Food and Bioproducts Processing 106 181–192 (2017)
  55. S. Nasser, A. Hédoux, A. Giuliani, C. Le Floch-Fouéré, V. Santé-Lhoutellier, I. De Waele, G. Delaplace, Investigation of secondary structure evolution of micellar casein powder upon aging by FTIR and SRCD: consequences on solubility, Journal of the Science of Food and Agriculture 149 105 (2017) [doi: 10.1002/jsfa.8711]
  54. T. Starciuc, Y. Guinet, L. Paccou, A. Hedoux, Influence of a Small Amount of Glycerol on the Trehalose Bioprotective Action Analyzed In Situ During Freeze-Drying of Lyzozyme Formulations by Micro-Raman Spectroscopy, Journal of Pharmaceutical Sciences 106 2988-2997 (2017) [doi: 10.1016/j.xphs.2017.05.040]
  53. S. Nasser, R. Jeantet, P. De-Sa-Peixoto, G. Ronse, N. Nuns, F. Pourpoint, J. Burgain, C. Gaiani, A. Hédoux, G. Delaplace, Microstructure evolution of micellar casein powder upon ageing: Consequences on rehydration dynamics, Journal of Food Engineering 206 57-66 (2017) [doi: 10.1016/j.jfoodeng.2017.03.004]
  52. Y. Guinet, L. Paccou, F. Danède, P. Derollez, A. Hédoux, Structural description of the marketed form of valsartan: A crystalline mesophase characterized by nanocrystals and conformational disorder, International Journal of Pharmaceutics (2017) [doi: 10.1016/j.ijpharm.2017.04.067]
  51. A. V. Frontzek (Neé Svanidze), J. P. Embs, L. Paccou, Y. Guinet, A. Hédoux, Low-Frequency Dynamics of BSA Complementarily Studied by Raman and Inelastic Neutron Spectroscopy, The Journal of Physical Chemistry B (2017) [doi: 10.1021/acs.jpcb.7b01395]
  50. A. Hédoux, Y. Guinet, L. Carpentier, L. Paccou, P. Derollez, S. A. Brandán, Structural and vibrational characterization of sugar arabinitol structures employing micro-Raman spectra and DFT calculations, Journal of Molecular Structure 1138 118-128 (2017) [doi: 10.1016/j.molstruc.2017.03.015]
• 2016
  49. Y. Guinet, L. Carpentier, L. Paccou, P. Derollez, A. Hédoux, Analysis of stochastic crystallization in micron-sized droplets of undercooled liquid l-arabitol, Carbohydrate Research 435 76-82 (2016) [doi: 10.1016/j.carres.2016.09.012]
  48. Y. Guinet, L. Paccou, F. Danède, J. F. Willart, P. Derollez, A. Hédoux, Comparison of amorphous states prepared by melt-quenching and cryomilling polymorphs of carbamazepine, International Journal of Pharmaceutics 509 305-313 (2016) [doi: 10.1016/j.ijpharm.2016.05.050]
  47. A. Hédoux, Recent developments in the Raman and infrared investigations of amorphous pharmaceuticals and protein formulations: A review, Advanced Drug Delivery Reviews 100 133-146 (2016) [doi: 10.1016/j.addr.2015.11.021]
  46. J. Junthip, N. Tabary, F. Chai, L. Leclercq, M. Maton, F. Cazaux, C. Neut, L. Paccou, Y. Guinet, J. N. Staelens, M. Bria, D. Landy, A. Hédoux, N. Blanchemain, B. Martel, Layer by layer coating of textile with two oppositely charged cyclodextrin polyelectrolytes for extended drug delivery, Journal of Biomedical Materials Research Part A 104 1408-1424 (2016) [doi: 10.1002/jbm.a.35674]
  45. L. Paccou, Y. Guinet, A. Hédoux, A. Frontzek, Study of the phase transition in lysozyme crystals by Raman spectroscopy, Biochimica et Biophysica Acta (BBA) - General Subjects 1860 412-423 (2016) [doi: 10.1016/j.bbagen.2015.10.020]
• 2015
  44. A. Hédoux, L. Paccou, P. Derollez, Y. Guinet, Dehydration mechanism of caffeine hydrate and structural description of driven metastable anhydrates analyzed by micro Raman spectroscopy, International Journal of Pharmaceutics 486 331-338 (2015) [doi: 10.1016/j.ijpharm.2015.04.001]
• 2014
  43. G. Bellavia, L. Paccou, Y. Guinet, A. Hédoux, How Does Glycerol Enhance the Bioprotective Properties of Trehalose? Insight from Protein–Solvent Dynamics, The Journal of Physical Chemistry B 118 8928-8934 (2014) [doi: 10.1021/jp500673b]
  42. A. Hédoux, L. Paccou, Y. Guinet, Relationship between β-relaxation and structural stability of lysozyme: Microscopic insight on thermostabilization mechanism by trehalose from Raman spectroscopy experiments, The Journal of Chemical Physics 140 225102 (2014) [doi: 10.1063/1.4882058]
• 2013
  41. A. Hedoux, L. Paccou, S. Achir, Y. Guinet, Mechanism of protein stabilization by trehalose during freeze-drying analyzed by in situ micro-raman spectroscopy, Journal of Pharmaceutical Sciences 102 2484-2494 (2013) [doi: 10.1002/jps.23638]
  40. G. Bellavia, L. Paccou, S. Achir, Y. Guinet, J. Siepmann, A. Hédoux, Analysis of Bulk and Hydration Water During Thermal Lysozyme Denaturation Using Raman Scattering, Food Biophysics 8 170-176 (2013) [doi: 10.1007/s11483-013-9294-3]
  39. A. Hédoux, Y. Guinet, L. Paccou, P. Derollez, F. Danède, Vibrational and structural properties of amorphous n-butanol: A complementary Raman spectroscopy and X-ray diffraction study, The Journal of Chemical Physics 138 214506 (2013) [doi: 10.1063/1.4808159]
  38. P. Derollez, A. Hédoux, Y. Guinet, F. Danède, L. Paccou, Structure determination of the crystalline phase ofn-butanol by powder X-ray diffraction and study of intermolecular associations by Raman spectroscopy, Acta Crystallographica Section B Structural Science Crystal Engineering and Materials 69 195-202 (2013) [doi: 10.1107/S0108768113004849]
  37. A. Lerbret, A. Hédoux, B. Annighöfer, M. C. Bellissent-Funel, Influence of pressure on the low-frequency vibrational modes of lysozyme and water: A complementary inelastic neutron scattering and molecular dynamics simulation study, Proteins: Structure, Function, and Bioinformatics 81 326-340 (2013) [doi: 10.1002/prot.24189]
  36. A. Hédoux, Y. Guinet, L. Paccou, F. Danède, P. Derollez, Polymorphic transformation of anhydrous caffeine upon grinding and hydrostatic pressurizing analyzed by low-frequency raman spectroscopy, Journal of Pharmaceutical Sciences 102 162-170 (2013) [doi: 10.1002/jps.23346]
• 2012
  35. P. Blanpain-Avet, A. Hédoux, Y. Guinet, L. Paccou, J. Petit, T. Six, G. Delaplace, Analysis by Raman spectroscopy of the conformational structure of whey proteins constituting fouling deposits during the processing in a heat exchanger, Journal of Food Engineering 110 86-94 (2012) [doi: 10.1016/j.jfoodeng.2011.12.005]
  34. A. Lerbret, F. Affouard, A. Hédoux, S. Krenzlin, J. Siepmann, M. C. Bellissent-Funel, M. Descamps, How Strongly Does Trehalose Interact with Lysozyme in the Solid State? Insights from Molecular Dynamics Simulation and Inelastic Neutron Scattering, The Journal of Physical Chemistry B 116 11103–11116 (2012) [doi: 10.1021/jp3058096]
  33. P. Derollez, Y. Guinet, F. Affouard, F. Danède, L. Carpentier, A. Hédoux, Structure determination ofL-arabinitol by powder X-ray diffraction, Acta Crystallographica Section B Structural Science 68 407-411 (2012) [doi: 10.1107/S0108768112019994]
  32. A. Hédoux, L. Paccou, S. Achir, Y. Guinet, In situ monitoring of proteins during lyophilization using micro-raman spectroscopy: A description of structural changes induced by dehydration, Journal of Pharmaceutical Sciences 101 2316-2326 (2012) [doi: 10.1002/jps.23172]
  31. P. Blanpain-Avet, A. Hédoux, Y. Guinet, L. Paccou, J. Petit, T. Six, G. Delaplace, Analysis by Raman spectroscopy of the conformational structure of whey proteins constituting fouling deposits during the processing in a heat exchanger, Journal of Food Engineering 110 86-94 (2012) [doi: 10.1016/j.jfoodeng.2011.12.005]
  30. A. Hédoux, J. A. Seo, Y. Guinet, L. Paccou, Analysis of cold denaturation mechanism of β-lactoglobulin and comparison with thermal denaturation from Raman spectroscopy investigations, Journal of Raman Spectroscopy 43 16-23 (2012) [doi: 10.1002/jrs.3009]
• 2011
  29. A. Hédoux, Y. Guinet, P. Derollez, E. Dudognon, N. T. Correia, Raman spectroscopy of racemic ibuprofen: Evidence of molecular disorder in phase II, International Journal of Pharmaceutics 421 45-52 (2011) [doi: 10.1016/j.ijpharm.2011.09.015]
  28. S. Hubert, S. Briancon, A. Hedoux, Y. Guinet, L. Paccou, H. Fessi, F. Puel, Process induced transformations during tablet manufacturing: Phase transition analysis of caffeine using DSC and low frequency micro-Raman spectroscopy, International Journal of Pharmaceutics 420 76-83 (2011) [doi: 10.1016/j.ijpharm.2011.08.028]
  27. A. Hédoux, Y. Guinet, L. Paccou, Analysis of the Mechanism of Lysozyme Pressure Denaturation from Raman Spectroscopy Investigations, and Comparison with Thermal Denaturation, The Journal of Physical Chemistry B 115 6740-6748 (2011) [doi: 10.1021/jp2014836]
  26. A. Hédoux, A. A. Decroix, Y. Guinet, L. Paccou, P. Derollez, M. Descamps, Low- and High-Frequency Raman Investigations on Caffeine: Polymorphism, Disorder and Phase Transformation, The Journal of Physical Chemistry B 115 5746-5753 (2011) [doi: 10.1021/jp112074w]
  25. A. Hédoux, Y. Guinet, M. Descamps, The contribution of Raman spectroscopy to the analysis of phase transformations in pharmaceutical compounds, International Journal of Pharmaceutics 417 17-31 (2011) [doi: 10.1016/j.ijpharm.2011.01.031]
  24. N. Dujardin, E. Dudognon, J. F. Willart, A. Hédoux, Y. Guinet, L. Paccou, M. Descamps, , Solid state mutarotation of glucose, J. Phys. Chem. B 115 1698-1705 (2011) [doi: 10.1021/jp109382j]
  23. A. Lerbret, F. Affouard, P. Bordat, A. Hédoux, Y. Guinet, M. Descamps, Slowing down of water dynamics in disaccharide aqueous solutions, Journal of Non-Crystalline Solids 357 695-699 (2011) [doi: 10.1016/j.jnoncrysol.2010.05.092 ]
• 2010
  22. A. Hédoux, S. Krenzlin, L. Paccou, Y. Guinet, M. P. Flament, J. Siepmann, Influence of urea and guanidine hydrochloride on lysozyme stability and thermal denaturation; a correlation between activity, protein dynamics and conformational changes, Physical Chemistry Chemical Physics 12 13189 (2010) [doi: 10.1039/C0CP00602E ]
  21. J. A. Seo, A. Hédoux, Y. Guinet, L. Paccou, F. Affouard, A. Lerbret, M. Descamps, Thermal Denaturation of Beta-Lactoglobulin and Stabilization Mechanism by Trehalose Analyzed from Raman Spectroscopy Investigations, Journal of Physical Chemistry B 114 6675 (2010) [doi: 10.1021/jp1006022]
• 2009
  20. A. Hédoux, L. Paccou, Y. Guinet, J. F. Willart, M. Descamps, Using the low-frequency Raman spectroscopy to analyze the crystallization of amorphous indomethacin, European Journal of Pharmaceutical Sciences 38 156 – 164 (2009) [doi: doi:10.1016/j.ejps.2009.06.007]
  19. A. Hédoux, J. F. Willart, L. Paccou, Y. Guinet, F. Affouard, A. Lerbret, M. Descamps, Thermostabilization mechanism of bovine serum albumine by trehalose, Journal of Physical Chemistry B 113 6119 (2009) [doi: 10.1021/jp900330r]
  18. A. Lerbret, F. Affouard, P. Bordat, A. Hédoux, Y. Guinet, M. Descamps, Low-frequency vibrational properties of lysozyme in sugar aqueous solutions: a Raman scattering and molecular dynamics simulation study, Journal of Chemical Physics 131 245103 (2009) [doi: 10.1063/1.3273218]
• 2008
  17. F. Affouard, A. Lerbret, A. Hédoux, Y. Guinet, M. Descamps, Biopreservative capabilities of disaccharides on proteins: A study by molecular dynamics simulations, AIP Conference Proceedings 982 690-695 (2008) [doi: 10.1063/1.2897879]
  16. N. Dujardin, J. F. Willart, E. Dudognon, A. Hédoux, Y. Guinet, L. Paccou, B. Chazallon, M. Descamps, Solid state vitrification of crystalline a and b-D-glucose by mechanical milling, Solid State Communications 148 78-82 (2008) [doi: 10.1016/j.ssc.2008.07.002]
  15. A. Hédoux, Y. Guinet, F. Capet, L. Paccou, M. Descamps, Evidence for a high-density amorphous form in indomethacin from Raman scattering investigations, Physical Review B 77 094205 (2008) [doi: 10.1103/PhysRevB.77.094205]
  14. A. Lerbret, F. Affouard, P. Bordat, A. Hédoux, Y. Guinet, M. Descamps, Molecular dynamics simulations of lysozyme in water/sugar solutions, Chemical Physics 345 267-274 (2008) [doi: 10.1016/j.chemphys.2007.09.011]
• 2007
  13. A. Hédoux, F. Affouard, M. Descamps, Y. Guinet, L. Paccou, Microscopic description of protein thermostabilization mechanisms with disaccharides from Raman spectroscopy investigations, Journal of Physics Condensed Matter 19 205142 (2007) [doi: 10.1088/0953-8984/19/20/205142]
  12. O. J. Hernandez, A. Boucekkine, A. Hédoux, Density functional theory study of triphenyl phosphite: Molecular flexibility and weak intermolecular hydrogen bonding, Journal of Physical Chemistry A 111 6952-6958 (2007) [doi: 10.1021/jp071934d]
  11. A. Lerbret, P. Bordat, F. Affouard, A. Hédoux, Y. Guinet, M. Descamps, How do trehalose, maltose, and sucrose influence some structural and dynamical properties of lysozyme? Insight from molecular dynamics simulations, Journal of Physical Chemistry B 111 9410-9420 (2007) [doi: 10.1021/jp071946z]
  10. A. Wypych, Y. Guinet, A. Hédoux, Isothermal transformation of supercooled liquid n -butanol near the glass transition: Polyamorphic transitions in molecular liquids investigated using Raman scattering, Physical Review B 76 144202 (2007) [doi: 10.1103/PhysRevB.76.144202]
• 2006
  9. P. Derollez, A. Hédoux, Y. Guinet, J. Lefebvre, M. Descamps, O. Hernandez, Micro(nano)structure of the glacial state in triphenyl phosphite (TPP), Zeitschrift fur Kristallographie Supplements 2006 557-562 (2006) [doi: 10.1524/zksu.2006.suppl_23.557]
  8. A. Hédoux, Y. Guinet, P. Derollez, O. Hernandez, L. Paccou, M. Descamps, Microstructural investigations in the glacial state of triphenyl phosphite, Journal of Non-Crystalline Solids 352 4994 (2006) [doi: 10.1016/j.jnoncrysol.2006.02.155]
  7. A. Hédoux, R. Ionov, J. F. Willart, A. Lerbret, F. Affouard, Y. Guinet, M. Descamps, D. Prévost, L. Paccou, F. Danède, Evidence of a two-stage thermal denaturation process in lysozyme: a Raman scattering and differential scanning calorimetry investigation, Journal of Chemical Physics 124 014703-014710 (2006) [doi: 10.1063/1.2139087]
  6. A. Hédoux, J. F. Willart, R. Ionov, F. Affouard, Y. Guinet, L. Paccou, A. Lerbret, M. Descamps, Analysis of sugar bioprotective mechanisms on the thermal denaturation of lysozyme from Raman scattering and differential scanning calorimetry investigations, Journal of Physical Chemistry B 110 22886-22893 (2006) [doi: 10.1021/jp061568i]
  5. R. Ionov, A. Hédoux, Y. Guinet, P. Bordat, A. Lerbret, F. Affouard, D. Prevost, M. Descamps, Sugar bioprotective effects on thermal denaturation of lysozyme: Insights from Raman scattering experiments and molecular dynamics simulation, Journal of Non-Crystalline Solids 352 4430-4436 (2006) [doi: 10.1016/j.jnoncrysol.2006.01.105]
  4. J. F. Willart, A. Hédoux, Y. Guinet, F. Danède, L. Paccou, F. Capet, M. Descamps, Metastability release of the alpha form of trehalose by isothermal solid state vitrification, Journal of Physical Chemistry B 110 11040-11043 (2006) [doi: 10.1021/jp061784r]
• 2005
  3. A. Lerbret, P. Bordat, F. Affouard, Y. Guinet, A. Hedoux, L. Paccou, D. Prevost, M. Descamps, Influence of homologous disaccharides on the hydrogen bond network of water: Complementary Raman scattering experiments and molecular dynamics simulations, Carbohydrate Research 340 881 (2005) [doi: 10.1016/j.carres.2005.01.036]
• 2004
  2. P. Derollez, O. Hernandez, A. Hedoux, Y. Guinet, O. Masson, J. Lefebvre, A. Descamps, Structural and microstructural description of the glacial state in triphenyl phosphite from powder synchrotron X-ray diffraction data and Raman scattering investigations, Journal of Molecular Structure 694 131-138 (2004) [doi: 10.1016/j.molstruc.2004.03.033]
  1. A. Hedoux, Y. Guinet, P. Derollez, O. Hernandez, R. Lefort, M. Descamps, A contribution to the understanding of the polyamorphism situation in triphenyl phosphite, Physical Chemistry Chemical Physics 6 3192-3199 (2004) [doi: 10.1039/b401262c]